Difficult to Express Proteins
Strategies for Solving “Finicky” Protein Problems
7-8 November 2013
Preliminary Agenda
This third part of the protein expression stream for PEGS Europe will focus on the “finicky” proteins that are extremely challenging to expression. Vaccines, hormones, membrane proteins, ion channels, low-abundance proteins and protein complexes continue to cause issues for bench scientists. This meeting will focus on the novel and imaginative solutions for these problems.
KEYNOTE PRESENTATION
Expression of Difficult Protein Therapeutics:
Bi-Specific Antibodies
Yves Durocher, Ph.D., Senior Research Officer, National
Research Council
of Canada
Total Protein Engineering Solution for GPCRs through
Stabilization and Construct Optimization
Markus Koglin, Ph.D., Associate Director, Protein Engineering,
Heptares Therapeutics
Directed Evolution of G Protein-Coupled Receptors for High
Functional Expression in E. coli and Stability in Detergents
Pascal Egloff, Ph.D., Plückthun Laboratory, University of
Zurich
Expression and Purification of Multiple Isoforms and
Phosphoforms of the Heterotrimeric Enzyme AMPK in Escherichia
coli
Isabelle Green, Ph.D., Reagents and Assay Development,
AstraZeneca, United Kingdom
Producing Human Multiprotein Complexes: New Tools and
Strategies
Imre Berger, Ph.D., Group Leader and Principal Investigator,
Structural Biology, EMBL Grenoble
Solving Issues of Difficult to Express Therapeutic Fusion
Proteins
Stefan Schmidt, Ph.D., Vice President, DSP Production,
Rentschler Biotechnology
Lactococcus lactis: An Alternative and Emerging System for
Functional Expression of Difficult Membrane Proteins
Annie Barrand-Frelet, CNRS Researcher, CEA Saclay/iBiTec-S
The Novel Fh8 Fusion Tag: A Comparison Screening with Other Fusion Systems for Soluble Overexpression and Purification of Recombinant Proteins in Escherichia coli
Sofia J. Costa, IBB - Institute for Biotechnology and
Bioengineering, Centre of Biological Engineering, University of Minho