High-Throughput Protein Expression
Screening, Miniaturization, and Engineering for Success
4-5 November 2013
Preliminary Agenda
The steps required to prepare an expression system prior to scale-up have been plagued with difficulties through the past decades. Methods for testing the system parameters, temperature, HT selection and fine-tuning of hosts, clones, culture media, containers and so on are constantly being introduced, and then go by the wayside as they fail to meet expectations. Novel methods and strategies are crucial to successfully prepare the elements needed. This first part of the protein expression stream for PEGS Europe will focus on the critical early stages of protein expression scale-up. We will focus on automation and robotics, assay development, selection strategies for clones, vectors and hosts, software, data mining for later success, single cell analysis, fine tuning growth characteristics, as well as other strategies and technologies to facilitate high-throughput method success.
Optimized Methods to Generate High-Quality ScFv Libraries and
Isolate IgG Candidates by High-Throughput Screening
Philippe Mondon, Ph.D., CTO, MILLEGEN SA
High-Throughput Approaches to Obtain Crystallizable Constructs
of Several Difficult Kinases for Structure-Based Design of Inhibitors
Jacques Dumas, Ph.D., Head, Protein Production, VitryBiologics
SCP, Vitry Research Center, Sanofi
High-Throughput Expression in Mammalian Cells
Trevor Wilkinson, Ph.D., Associate Director, Protein Sciences,
MedImmune, Ltd.
Insights into How to Design Assays and Correctly Plan for
Candidate Profiling in the Most Cost-Effective, Rapid and Dynamic way
David O’Connell, Ph.D., Director of MSc. Programs in
Biotechnology, School of Biomolecular & Biomedical Research, University
college Dublin; Conway Institute of Biomolecular & Biomedical Research, UCD
Belfield
Automation Platforms for Bacterial and Mammalian Protein
Expression Systems
Scott Lesley, Ph.D., Director, Protein Sciences and
Biotherapeutics, Genomics Institute of the Novartis Research Foundation
FX Cloning: A Versatile High-Throughput Cloning System for
Characterization of Enzyme Variants
Eric R. Geertsma, Ph.D., Department of Biochemistry,
University of Zurich, Zurich, Switzerland
High Throughput Construction and Small Scale Expression
Screening of Multi-Tag Vectors in Escherichia coli
Louise E. Bird, Ph.D., Division of Structural Biology, Henry
Wellcome Building for Genomic Medicine, University of Oxford
High-Throughput Analytics
Speaker TBA, Malvern Instruments
Screening for High-Yielding Pichia
pastoris Clones: The Production of G-Protein Coupled Receptors as a Case
Study
Bernadette Byrne, Ph.D., Professor, Division of Molecular
Biosciences, Imperial College London
Automating the Ribosome Display Technology and High-Throughput
Screening
Jonas Schaefer, Ph.D., University of Zurich
Fractional Factorial Approach Combining 4 Escherichia coli Strains, 3 Culture Media, 3 Expression
Temperatures and 5 N-Terminal Fusion Tags for Screening the Soluble Expression
of Recombinant Proteins
Bruno Coutard, Ph.D. Architecture et Fonction des
Macromolécules Biologiques (AFMB), UMR CNRS and Aix-Marseille University