Optimizing Protein Expression
Driving Results with Smarter Strategies
6-7 November 2013
Preliminary Agenda
The expression of proteins for characterization and therapeutics has grown dramatically over the last 2 decades. However, the higher demand for larger amounts of protein, in shorter periods of time continue to plague protein researchers. It is crucial to increase the throughput of expression, production and purification processes. Sometimes this requires new technologies and strategies, other times it requires a re-working or re-engineering of existing resources to improve outcomes. In addition, success demands making wise and effective choices in the selection of the host for protein expression. This second part of the protein expression stream for PEGS Europe will focus on the methods and technologies for achieving the highest possible yield, with the best functionality.
Toward System-Level Understanding of Baculovirus-Host Cell
Interactions: From Molecular Fundamental Studies to Large-Scale Proteomics
Approaches
Paula M. Alves, Ph.D., Director, Animal Cell Technology Unit,
Instituto de Biologia Experimental e Tecnológica Oeiras, Portugal
Optimizing Heterologous Protein Production in the Periplasm of
E. coli
Jan-Willem de Gier, Ph.D., Associate Professor, Center for
Biomembrane Research, Stockholm University; CSO, Xbrane Bioscience AB
Use of an Anti-Apoptotic CHO Cell Line for Transient Gene
Expression
Athena Wong, Ph.D., Scientist, Early Stage Cell Culture,
Genentech
Generation and Optimization of a Suspension CHO-EBNA
Expression System to Express Multiple Different Classes of Proteins
Robert Roth, Ph.D., Reagents and Assay Development,
AstraZeneca, Sweden
GlycoDelete Technology: Shortcutting Mammalian Cell
N-glycosylation to Produce Therapeutic Proteins with Simplified Sialylated
N-glycans
Francis Santens, Researcher, Department for Molecular
Biomedical Research, VIB
Mammalian Stable Expression of Biotherapeutics
Thomas Jostock, Ph.D., Novartis Pharma AG, Basel
Production of Minicircles for Gene Therapy and Transient
Protein Expression
Michaela Simcikova, Center for Biological and Chemical
Engineering, IBB-Institute for Biotechnology and Bioengineering, Instituto
Superior Técnico