PEGS Europe 2010 | Day 1 | Day 2
A conference that examines this promising new generation of biotherapeutic from cell line selection to creation of libraries, screening and beyond. Industry and academic experts present antigen-binding, potency and ability to reach difficult targets together with in vitro and in vivo pre-clinical investigations and, in some cases, regulatory approval and further clinical study. Characterization and imaging of target binding are included as well as key features such as stability, half-life, and resistance to aggregation and post-translational modification, and application to specialized delivery.
TUESDAY, 5 OCTOBER
9:00 Conference Registration and Morning Coffee
9:30 Chairperson’s Opening Remarks
Christian Heinis, Ph.D.,Laboratory of Therapeutic Proteins and Peptides, Institute of Chemical Sciences and Engineering, Ecole Polytechnique Fédérale de Lausanne (EPFL)
9:35 Modular Antibodies: Introducing Antigen-Binding Sites in the Fc Region of IgG
Max Woisetschläger, Ph.D., Director, Target Biology, f-star Biography
We have developed two novel antibody formats: Fcabs, in which antigen-binding sites are introduced into a human Fc fragment; and mAb2s, in which additional binding sites are engineered into the Fc of an intact antibody. Fcabs allow small therapeutic antibody fragments to be isolated that retain all normal antibody functionalities (antigen binding, effector functions and long half life) while mAb2s represents an elegant way to create bispecific antibodies. Examples will be described demonstrating the potential of these proteins as next generation therapeutic biologicals.
10:05 IL-17 Neutralizing Fynomers: Making Use of Fc Fusion Proteins
Dragan Grabulovski, Ph.D., Chief Scientific Officer, Covagen, A.G. Biography
We describe the design, construction, characterization, and use of a large human Fyn SH3 library comprising 8.5 x 10e10 individual clones (termed Fynomers). The versatility and broad applicability of the Fynomer technology will be presented as well as the in vitro and in vivo characterization of high-affinity Fynomers binding to IL-17. We will demonstrate how engineered Fynomer-Fc fusion proteins having appropriate physico-chemical and in vivo half-life properties are attractive drug candidates for pre-clinical and clinical development.
10:35 Coffee Break
11:00 Exploiting the Biophysical Properties of Centyrins for Alternative Routes of Delivery
Steve Jacobs, Ph.D. Principal Scientist, Centyrex Venture, Johnson & Johnson, Inc. Biography
Alternative scaffolds represent an emerging of protein drugs that combine the attractive specificity properties of mAbs with the simplicity, ease of manufacture and tissue penetration associated with small molecules. We are exploiting the properties of an exceptionally stable alternative scaffold to develop a series of molecules tailored for alternative routes of delivery. The development and application of the Centyrin technology will be presented, together with in vitro and in vivo characterization of high-affinity Centyrins against an array of therapeutic targets.
11:30 Anticalins: A Differentiated Biologics Drug for Novel Delivery, Broad Target Space and New Modes of Action
Kristian H. Jensen, Ph.D., Chief Operating Officer, Pieris AG Biography
Anticalins are modified versions of human lipocalins. Pieris’ lead project PRS-050 (VEGF antagonist) is now entering human studies. Unique features of this drug such as the ability to bind therapeutically relevant hapten targets will be presented with their broad formulation and delivery options and formatting flexibility.
12:00 Glycan-Binding Decoy Proteins with Strong Anti-Inflammatory Activity Derived from the CellJammer® Platform Technology
Andreas Kungl, CSO, ProtAffin Biotechnologie AG
ProtAffin is developing novel biopharmaceuticals targeting protein-glycan interactions in inflammation. The importance of especially glycosaminoglycans in regulating protein function has been under-exploited as a basis of therapeutics for a long time mainly due to the complexity of this glycan class which prevents easy chemical synthesis. ProtAffin’s CellJammer® discovery platform takes advantage of a protein’s natural glycan-binding function to improve it thereby generating unique decoy proteins with anti-inflammatory, anti-metastatic or anti-angiogenic activity.
12:30 Lunch for Purchase in the Exhibit Hall
13:45 Dedicated Poster Viewing in the Exhibit Hall
14:30 Chairperson’s Remarks
Josefin-Beate Holz, Chief Medical Officer, Ablynx NV
14:35 From Clinical Imaging to Serum Half-Life Extension Using HER2-Specific Affibody Molecules
Fredrik Y. Frejd, Ph.D., Project Manager, Biotherapeutics, Affibody AB Biography
Affibody molecules are 6.5-7 kDa scaffold proteins with rapid in vivo kinetics. Pre-clinical data leading to clinical trial regulatory approval for a HER2-targeting Affibody imaging agent will be presented. The half life and distribution profile of the imaging molecule was enhanced using albumin binding technology, allowing for successful targeted radionuclide therapy in xenografted mice. Furthermore, the albumin binding technology is general, as shown in a pharmacodynamic study in rats using modified G-CSF associating with albumin.
15:05 PASylation: A Superior Technology to Extend the Plasma Half-Life of Therapeutic Proteins
Arne Skerra, Ph.D., CEO, XL-protein GmbH Biography
PAS sequences form conformationally disordered biological polymers with large hydrodynamic volume and high solubility, similar to PEG. In contrast, PASylated biopharmaceuticals can be directly produced in microbial expression systems with a wide range of therapeutic proteins, thus avoiding costly and laborious chemical modification steps. PAS sequences can prolong the pharmacokinetics of biologics in mice by a factor of 10-100. Preclinical data for several pharmaceutically attractive protein drug classes, including antibody fragments and alternative scaffolds, will be presented.
15:35 Refreshment Break
16:00 Sponsored Presentation (Opportunity Available)
16:30 Tn3: A New Platform for Non-antibody Protein Drugs
Manuel Baca, Ph.D., Principal Scientist, Antibody Discovery and Protein Engineering, MedImmune, Inc. Biography
We have developed an alternate scaffold platform from a small Ig-like protein module. This scaffold can be expressed at high levels in bacteria and has been engineered for high stability. Sequence and length diversification of surface loops has been used to create large diversity libraries from which target binders can be readily selected. This presentation will describe the unique features of this platform and its flexibility in engineering format and valency for next generation therapeutics.
17:00 Generating A Best-in-Class DARPin Therapeutic for the Treatment of Ophthalmic Neovascularization Diseases
H. Kaspar Binz, Ph.D., Vice President Technology, Molecular Partners AG Biography
DARPins combine the advantages of antibodies with those of small molecule drugs and allow us to generate a broad pipeline of innovative new drug candidates in a short time. We will present hands-on data on how we choose the best of the many drug candidates and how we assay them in vitro and in vivo. This process will be illustrated by the example of a best-in-class therapeutic DARPin for the treatment of wet AMD and other ocular neovascularization diseases.
17:30 Challenges in Domain Antibody Development
Thomas Sandal, Head, Microbial Process Research, GlaxoSmithKline Research & Development Biography
GSK has developed an innovative approach for assessing the development of potential novel domain antibodies (dAbs). The strategy comprises high-throughput and downscaled methods to evaluate the biophysical properties and purification challenges for a range of dAbs. In order to develop a commercially viable expression system, a high-throughput method has been implemented to screen a combination of recombinant vector designs and host strains. This high-throughput screening technique facilitates the selection of a production strain and vector based on the productivity and quality
18:15 Interactive Breakout Discussion Groups
19:15 – 21:00 CHI Networking Reception