Mary Ruberry from CHI interviews Oliver Spadiut from the Vienna University of Technology on PAT strategies and production with yeast

Speaker at PEGS Europe’s “Bioproduction: Scaling Up & Down” Conference in Lisbon, Portugal, November 5-6, 2015

Oliver_SpadiutOliver Spadiut, Ph.D., Group Leader, Integrated Bioprocess Development, Biochemical Engineering, Vienna University of Technology


How do you employ PAT (Process Analytical Technology) tools in bioprocess monitoring?

We use an online HPLC system to follow impurity release during upstream processing, but also follow the impurity pattern during downstream processing. We employ our PAT tool across unit operations to allow for integrated bioprocess development.

How do you use monolithic columns as efficient impurity monitoring tools during bioreactor cultivations?

We use monoliths in HPLC format to measure product, protein, RNA and DNA in the cultivation broth as well as along the whole bioprocess. We employ an online HPLC system to allow monitoring quasi in real time.

Can you describe how you monitor quality of unit operations for recombinant protein production processes with yeast?

Yeasts have the tendency of hyperglycosylating the recombinant protein products. Thus, we can distinguish between products and host cell proteins and can thus follow the success of single unit operations by analyses of chromatograms.

Is there anything you’d like to add?

PAT tools are a prerequisite when it comes to the realization of continuous Bioprocessing.